Thioltracker violet fluorescent dye
WebFor GSH levels, the cells were stained with ThiolTracker™ Violet fluorescent probe according to the protocol at 37 °C for 30 min and then washed. The images were acquired by CLSM or the cells were digested and loaded on flow cytometry. Quantitative data were obtained with ImageJ. WebFurthermore, differences in GSH levels in response to oxidative stress in subpopulations of cells have been reported, underscoring the importance of detection methods amenable to flow cytometry and automated fluorescence detection. ThiolTracker™ Violet can be used for Flow Cytometry, HCS imaging, and epifluorescent microscopy.
Thioltracker violet fluorescent dye
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WebThiolTracker Violet dye is well excited with 405 nm laser or non-laser based light sources for fluorescence microscopy. Wavelength (nm) Absorption. Fluorescence emission. Absorption. Absorption. Fluorescence emission. Fluorescence emission. Labeling procedure. GSH is a major intracellular target of ThiolTracker Violet dye . U-2 OS cells … WebFeb 1, 2014 · We have carried out staining in three ecdysterone-treated (0, 250, 500, and 750 μM, 48 h) breast cancer cell lines with LysoTracker, a fluorescent dye, reflecting the number of lysosomes which...
WebBioAssay record AID 1414236 submitted by ChEMBL: Hepatotoxicity in human HepaRG cells assessed as GSH depletion at 500 uM after 3 to 12 hrs by ThiolTracker Violet dye-based fluorescence assay. WebMar 13, 2024 · In pure water at 25°C the hydronium-ion concentration is close to 1.00 × 10 –7 mol dm –3, so that the pH is 7.In consequence any solution, not only pure water, which …
WebThiolTracker Violet Basic Info Spectra Quantum Yields Lifetimes Availabilities Applications Basic Information Common name (s): ThiolTracker Violet IUPAC name: CAS number: …
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WebAfter loading into cells, dye is well retained, allowing for multigenerational tracking of cellular movements Violet excitation/emission spectra (415/516nm maxima) are optimal for multiplexing with green and red fluorescent dyes and proteins Easy to use: remove medium, add dye, incubate 30 minutes, and image cells fl civics eoc practice testWebAbsorption Fluorescence emission GSH is a major intracellular target of ThiolTracker Violet dye - BSO + ThiolViolet ThiolViolet- GSH MW Std. 17 kD 6 kD 3 kD • U-2 OS cells (human, … cheesecake factory austin tx menuWebFig. 2. Examples of improved spectral characteristics of StarBright Dyes. Reduced excitation of StarBright Blue 700 Dye, compared with PerCP-Cy5.5 (red circle), and reduced excitation by the 355 nm laser with narrow emission for StarBright Violet 515 Dye, compared to Brilliant Violet 510 (blue circles). flckr paladin27 emilyWebThiolTracker™ Violet is a brighter and more robust intracellular thiol probe for reproducible GSH detection. Since reduced glutathione represents the majority of intracellular free … ThiolTracker™ Violet is a brighter and more robust intracellular thiol probe for … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes cheesecake factory ayrWebJul 1, 2010 · The glutathione content ( Figure 5A) of the viable cells was determined after 4 h ( Figure 5B) and 24 h ( Figure 5D) using the fluorescent dye ThiolTracker Violet, which … cheesecake factory aventura reservationsWebAn illustrative fluorescence image of ThiolTracker Violet distribution in U87 MG cells is presented in the upper left panel. The cells were subjected to 5 μM insulin fibrils, and Ce1 and Ce5 ... fl civil rights act of 1992WebApr 9, 2024 · Lagos, city and chief port, Lagos state, Nigeria. Until 1975 it was the capital of Lagos state, and until December 1991 it was the federal capital of Nigeria. Ikeja replaced … fl city water